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目的 研究动脉硬化性脑梗死 (ACI)患者中的载脂蛋白E(ApoE)基因的分布 方法 利用聚合酶链式反应 (PCR)技术扩增ApoE基因含编码 112位和 15 8位氨基酸的片段 ,并用限制性片段长度多态性 (RFLP)技术对ACI患者和相应健康对照的ApoE基因进行分型 ,从而进行ACI与ApoE等位基因多态性的关联分析 .结果 ACI患者中ApoE等位基因ε4占 2 8.30 % ,明显高于正常人对照组的 7.6 4% ,而等位基因ε3则占 5 7.5 5 % ,低于正常人对照组的84.12 % ,均有极显著性差异 (p<0 .0 1) .结论 PCR -RFLP是一种快速有效的ApoE基因分型方法 ,ε4可能为ACI的易感因子 ,而ε3则为保护因子 .
Objective To study the distribution of apolipoprotein E (ApoE) gene in patients with arteriosclerotic cerebral infarction (ACI). ApoE gene was amplified by polymerase chain reaction (PCR) The ApoE gene of ACI patients and corresponding healthy controls were genotyped by restriction fragment length polymorphism (RFLP) to analyze the association between ACI and ApoE alleles.Results The ApoE allele ε4 Accounting for 2. 8.30%, significantly higher than the control group of normal people 7.64%, while the allele ε3 accounted for 7.55%, lower than the 84.12% of the normal control group, were significantly different (p <0. 0 1) .Conclusion PCR-RFLP is a rapid and effective ApoE genotyping method, ε4 may be a susceptibility factor of ACI, and ε3 is a protective factor.