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目的分析人类免疫缺陷病毒(HIV-1)急性期感染者(acute HIV-1-infected patients,AHIs)肠道归巢α4β7 Th17细胞的变化特点。方法本研究共纳入26例HIV-1急性期感染者、20例HIV-1慢性期感染者和20例健康对照者。采用流式细胞仪检测肠道归巢α4β7Th17细胞的比例和数量,并分析肠道归巢α4β7Th17细胞与CD4~+T细胞、T细胞免疫活化水平之间的相关性。结果与健康对照者比较,HIV-1急性期感染者肠道归巢α4β7Th17细胞的比例(2.61%vs 1.34%,P<0.000 1)和数量(2.29 vs 0.66,P<0.000 1)均显著减少,肠道归巢α4β7 Th17细胞与肠道归巢α4β7 CD4~+T细胞(r=0.42,P=0.02)和CD4~+T细胞(r=0.60,P=0.002)呈显著正相关。HIV-1急性期感染导致T细胞免疫活化水平升高(1.87%vs 15.60%,P<0.000 1),肠道归巢α4β7 Th17细胞与T细胞免疫活化水平呈负相关(r=-0.47,P=0.01)。结论肠道归巢α4β7 Th17细胞的丢失可能在HIV-1致病机制中起作用。
Objective To analyze the change characteristics of α4β7 Th17 cells in homing of human immunodeficiency virus (HIV-1) infected acute HIV-1-infected patients (AHIs). Methods A total of 26 HIV-1 infected patients, 20 chronic HIV-1 infected patients and 20 healthy controls were enrolled in this study. The proportion and quantity of α4β7Th17 cells homing in the gut were detected by flow cytometry. The correlation between the α4β7Th17 cells and the immune activation of CD4 ~ + T cells and T cells was analyzed. Results Compared with healthy controls, the percentage of α4β7Th17 cells (2.61% vs 1.34%, P <0.000 1) and the number (2.29 vs 0.66, P <0.000 1) of intestinal homing in HIV-1 acute phase were significantly decreased There was a significant positive correlation between α4β7 Th17 cells homing to colon and hCα4β7 CD4 ~ + T cells (r = 0.42, P = 0.02) and CD4 ~ + T cells (r = 0.60, P = 0.002) Acute phase infection with HIV-1 resulted in an increase in T cell activation (1.87% vs 15.60%, P <0.0001), and a negative correlation between α4β7 Th17 cells and T cell immune activation (r = -0.47, P = 0.01). Conclusion The loss of α4β7 Th17 cells in the homing of the intestine may play a role in the pathogenesis of HIV-1.