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目的研究氨基胍对糖尿病肾组织内糖化终产物（ＡＧＥｓ）受体（ＲＡＧＥ）ｍＲＮＡ表达的影响。方法采用逆转录聚合酶链式反应（ＲＴＰＣＲ）检测病程８周肾内ＲＡＧＥｍＲＮＡ水平。结果诱发糖尿病４周后糖尿病大鼠肾皮、髓质内ＲＡＧＥｍＲＮＡ表达增加（Ｐ＜０．０５），８周时更为明显（Ｐ＜０．０５）；口服补充氨基胍４周对肾内这种改变未产生影响（Ｐ＞０．０５），给药８周，糖尿病大鼠肾皮、髓质内增强的ＲＡＧＥｍＲＮＡ表达明显缓解（Ｐ＜０．０５）。与此同时，氨基胍在不影响血糖的基础上，使糖尿病大鼠升高的糖化血红蛋白（ＧＨｂ）水平下降２６．７２％。结论糖尿病状态下，肾组织内ＲＡＧＥｍＲＮＡ高表达可能是高血糖诱发ＡＧＥｓ形成的结果；氨基胍可通过调节ＲＡＧＥｍＲＮＡ的异常表达，缓解ＡＧＥｓ对肾组织的损伤。
Objective To investigate the effect of aminoguanidine on the expression of AGEs receptor (RAGE) mRNA in diabetic rats. Methods RT-PCR was used to detect the level of renal RAGEmRNA in the course of 8 weeks. Results After 4 weeks of diabetes mellitus, the expression of RAGEmRNA in renal cortex and medulla increased (P <0.05) in diabetic rats 4 weeks and more significantly at 8th week (P <0.05). After 4 weeks of oral administration of aminoguanidine, (P> 0.05). After 8 weeks’ administration, the expression of RAGEmRNA in renal cortex and medulla increased significantly in diabetic rats (P <0.05). At the same time, aminoguanidine reduced the level of elevated glycated hemoglobin (GHb) in diabetic rats by 26.72% on the basis of not affecting blood glucose. Conclusion High expression of RAGEmRNA in diabetic kidney tissue may be the result of AGEs induced by hyperglycemia. Aminoguanidine can alleviate the damage of AGEs to renal tissue by regulating the abnormal expression of RAGEmRNA.