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目的 通过静脉注射异基因脾细胞和抗H 2 b 单抗加腹腔注射环磷酰胺诱导成年B6小鼠对异基因皮肤移植物产生免疫耐受。方法 经C5 7BL/ 6 (H 2 b,B6 )小鼠尾静脉注射BALB/c小鼠 (H 2 d)脾细胞 ,2d后腹腔注射环磷酰胺 (CP) ,随后 2次尾静脉注射抗小鼠的H 2 b 单克隆抗体 ,然后进行皮肤移植 ,并于耐受 30d对受体B6小鼠作混合淋巴细胞反应 (mixedlymphocytereaction ,MLR)、迟发型超敏反应 (delayedtypehypersensitivity,DTH)等耐受状态检测。结果 BALB/c小鼠的皮肤移植物在耐受B6小鼠中存活期特异延长。MLR和DTH检验证明 :B6小鼠对BALB/c小鼠的脾细胞产生特异性耐受 ,对无关第 3者KM小鼠的脾细胞仍表现出强烈的免疫反应。结论 抗MHC Ⅰ类分子单克隆抗体可明显促进“细胞 +CP”诱导的异基因皮肤移植耐受 ;克隆排除是诱导耐受的主要因素
Objective To induce immune tolerance of allogeneic skin grafts in adult B6 mice by intravenous injection of allogeneic splenocytes and anti-H 2 b monoclonal antibody plus intraperitoneal injection of cyclophosphamide. Methods Splenocytes of BALB / c mice (H 2 d) were injected via the tail vein of C5 7BL / 6 (H 2 b, B 6) mice and injected intraperitoneally with cyclophosphamide (CP) 2 days later. Mouse H 2 b monoclonal antibody, and then transplanted into the skin. The mice were subjected to mixed lymphocyte reaction (MLR) and delayed type hypersensitivity (DTH) tolerance in recipient B6 mice for 30 days. Detection. Results Skin grafts from BALB / c mice were specifically prolonged survival in resistant B6 mice. MLR and DTH tests demonstrated that B6 mice were specifically tolerant to spleen cells of BALB / c mice and still showed strong immune responses to splenocytes of unrelated third-party KM mice. Conclusions Anti-MHC class I monoclonal antibody can obviously promote the tolerance of allogeneic skin grafts induced by “cells + CP”. Clone exclusion is the main factor inducing tolerance