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目的 研究P16/MTSl与P21/WAF1/CIP1基因在子宫内膜癌发生、发展中的作用及其临床意义。方法 采用SP免疫组化技术对45例子宫内膜癌组织,15例子宫内膜不典型增生组织,18例正常子宫内膜组织进行P16和P21蛋白表达的检测。结果 正常内膜组织P16与P21蛋白均呈阳性表达,15例增生内膜组织中,P16阳性为12例,P21阳性为9例。45例子宫内膜癌组织中,P16阳性为29例,P21阳性为16例,共同阳性为13例;P16蛋白表达与内膜癌的病理分级有关,与临床分期及预后也有关,但无统计学意义;P21蛋白表达与肌层浸润深度、淋巴结转移及预后有极大的相关性,P16与P21蛋白在内膜癌中的表达有极明显相关性。结论 P16和P21/WAFl与子宫内膜癌的发生、发展有关。二基因可作为临床预后评估指标。
Objective To investigate the role of P16 / MTS1 and P21 / WAF1 / CIP1 in the pathogenesis and progression of endometrial carcinoma and its clinical significance. Methods The expression of P16 and P21 proteins in 45 cases of endometrial carcinoma, 15 cases of endometrial dysplasia and 18 cases of normal endometrium were detected by SP immunohistochemistry. Results The positive expression of P16 and P21 in normal endometrium was observed. Among 15 cases of proliferative endometrial tissue, 12 were positive for P16 and 9 were positive for P21. In 45 cases of endometrial carcinoma, P16 was positive in 29 cases, P21 was positive in 16 cases and positive in 13 cases. The expression of P16 protein was related to the pathological grade of endometrial carcinoma, but not related to clinical stage and prognosis Significance of P21 protein expression and depth of myometrial invasion, lymph node metastasis and prognosis have a very significant correlation, P16 and P21 protein expression in endometrial cancer has a very significant correlation. Conclusions P16 and P21 / WAF1 are associated with the occurrence and development of endometrial carcinoma. Two genes can be used as clinical prognostic indicators.