某些植物毒素有极强的毒力,能抑制真核细胞的蛋白质合成,蓖麻毒蛋白(Ricin)就是其中之一。如将单克隆抗体与毒素相结合,则有可能对靶细胞产生特异性的定向杀伤作用。这种毒素与抗体的结合物可称之为“免疫毒素”。 在“免疫毒素”的研究中,首先需要建立一种简便方法以获得大量的纯化蓖麻毒蛋白。文献中报告过各种提纯蓖麻毒蛋白的方法,其中较好的是离子交换层析法和亲和层析法。但在实际应用中,发现每次提取过程的可重复性和每批产品的纯度不够稳 Some plant toxins are extremely virulent and inhibit protein synthesis in eukaryotes, one of which is Ricin. If the combination of monoclonal antibodies and toxins, it is possible to target cells have a specific targeted killing. This combination of toxins and antibodies can be called “immunotoxins.” In the study of “immunotoxins”, it is first necessary to establish a convenient way to obtain a large amount of purified ricin. Various methods of purifying ricin have been reported in the literature, of which ion exchange chromatography and affinity chromatography are preferred. However, in practical applications, it was found that the reproducibility of each extraction process and the purity of each batch of products are not stable enough