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目的：研究白血病抑制因子（ＬＩＦ）对胚胎发育的影响。方法：用小鼠的单细胞胚胎体外培养１２０ｈ，观察不同浓度的ＬＩＦ对早期胚胎发育的影响。结果：①０．１ｎｇ／ｍｌ、１ｎｇ／ｍｌ和１０ｎｇ／ｍｌ浓度的ＬＩＦ对小鼠早期胚胎的发育和胚泡的形成有促进作用，０．０１ｎｇ／ｍｌ和１００ｎｇ／ｍｌ浓度的ＬＩＦ对胚胎的发育无明显影响；②ＬＩＦ对小鼠早期胚胎发育的影响主要发生于桑椹胚和胚泡期，对１～８细胞期胚胎无明显影响。结论：适当浓度的ＬＩＦ可促进胚胎发育和胚泡形成。
Objective: To study the effect of leukemia inhibitory factor (LIF) on embryonic development. Methods: Single-cell mouse embryos were cultured in vitro for 120 hours to observe the effect of different concentrations of LIF on early embryonic development. Results: (1) LIF at concentrations of 0.1ng / ml, 1ng / ml and 10ng / ml could promote early embryo development and blastocyst formation in mice. LIF at 0.01ng / ml and 100ng / ml could inhibit embryonic development No significant effect; (2) The effect of LIF on early embryo development in mice mainly occurred in morula and blastocyst stage, but had no significant effect on embryos in 1 ~ 8 cell stage. Conclusion: LIF at appropriate concentration can promote embryonic development and blastocyst formation.