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目的 :观察L 精氨酸 (L Arg)脂质体对慢性低氧高二氧化碳性肺动脉高压、右心室肥大大鼠的影响并探讨其机制。方法 :将 4 0只健康雄性SD大鼠随机分为 4组 :正常对照组 (NC组 )、低氧高二氧化碳 4w组 (HH组 )、低氧高二氧化碳加L Arg 4w组 (LA组 )和低氧高二氧化碳加L Arg脂质体 4w组 (LL组 )。采用化学比色法测定肺组织匀浆eNOS活性 ,用液体闪烁仪测定 [3 H] 瓜氨酸的生产量 ,计算肺动脉总一氧化氮合酶 (tNOS)的活性 ,组织原位杂交观测肺细小动脉eNOSmRNA的表达变化。结果 :①HH组平均肺动脉压力(mPAP)和右心室重量与左心室加室间隔重量比 (RV/LV +S)均高于NC组 ,LL组均明显低于LA组与HH组。②HH组的肺组织匀浆tNOS含量明显高于NC组 (P <0 .0 1) ,LL组高于LA组 (P <0 .0 5 ) ,而LA组与HH组无明显差异。③HH组的肺动脉tNOS活性显著高于NC组 (P <0 .0 1) ,而LL、LA、HH三组的tNOS活性之间均无明显差异 (P >0 .0 5 )。④HH组的肺细小动脉eNOSmRNA的平均吸光度值低于NC组 (P <0 .0 5 ) ,LA组高于HH组 (P <0 .0 5 ) ,而LL组明显高于HH组和LA组 (P均 <0 .0 1)。结论 :L Arg脂质体较L Arg有更明显的降低慢性低氧高二氧化碳大鼠肺动脉压和减轻右室重构的作用 ,其机制可能与L Arg脂质体促进L Arg的跨膜转运有关。
Objective: To observe the effect of L-arginine (L Arg) liposome on chronic hypoxic-hypercapnic pulmonary hypertension and right ventricular hypertrophy in rats and its mechanism. Methods: Forty healthy male Sprague Dawley rats were randomly divided into 4 groups: normal control group (NC group), hypoxic hypercapnic 4w group (HH group), hypoxic hypercapnic carbon dioxide plus L Arg 4w group (LA group) and Hypoxic hypercapnia with L Arg liposome 4w group (LL group). The lung tissue homogenate eNOS activity was measured by chemical colorimetric method. [3H] citrulline production was measured by liquid scintillation counting apparatus, and the activity of total nitrogen monoxide synthase (tNOS) in pulmonary artery was calculated. Arterial eNOS mRNA expression changes. Results: (1) The mean pulmonary arterial pressure (mPAP) and right ventricular weight (RV / LV + S) in HH group were significantly higher than those in NC group and LL group were significantly lower than those in LA group and HH group. ② The content of tNOS in lung homogenate of HH group was significantly higher than that of NC group (P <0.01), LL group was higher than that of LA group (P <0.05), but there was no significant difference between LA group and HH group. (3) The pulmonary artery tNOS activity in HH group was significantly higher than that in NC group (P <0.01). There was no significant difference in tNOS activity among LL, LA and HH groups (P> 0.05). The mean absorbance value of eNOS mRNA in pulmonary arterioles in HH group was lower than that in NC group (P <0.05), LA group was higher than HH group (P <0.05), while LL group was significantly higher than HH group and LA group (P <0.01). CONCLUSION: L Arg liposomes have a marked effect on reducing pulmonary arterial pressure and alleviating right ventricular remodeling in rats with chronic hypoxia and hypercapnia compared with L Arg, which may be related to L Arg liposomes promoting transmembrane transport of L Arg .