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细胞色素氧化酶 (CytochromeOxidase ,CO)是呼吸链上的最后一个酶 ,是细胞能量产生和传递的关键组成部分。在中枢神经系统中 ,区域性CO活性反映了局部组织能量代谢变化 ,这种变化与神经元的功能尤其是神经元突触和自发性电活动的能量需求密切相关。CO的组织化学染色方法已被广泛用于反映中枢神经系统CO的活性。为了成功地进行CO的组织化学染色 ,适中的组织固定是关键。固定剂的组成必须考虑既要保持良好的组织结构又要保持较高的酶活性。通常使用多聚甲醛和戊二醛混合固定液。本实验选用不同浓度的多聚甲醛和戊二醛固定组织 ,做CO组织化学染色 ,并进行光镜和电镜观察 ,发现 1%多聚甲醛和 2 %戊二醛混合固定既可保存良好的组织结构又可获得较高的CO活性。
Cytochrome Oxidase (CO) is the last enzyme on the respiratory chain and is a key component of cellular energy production and delivery. In the central nervous system, regional CO activity reflects changes in the energy metabolism of the local tissue, and this change is closely related to the neuronal function, especially the energy requirements of neuronal synapses and spontaneous electrical activity. Histochemical staining of CO has been widely used to reflect CO activity in the central nervous system. Moderate tissue fixation is the key to successful CO histochemical staining. The composition of the fixative must take into account the need to maintain both good tissue structure and high enzyme activity. Usually use paraformaldehyde and glutaraldehyde mixed fixative. In this experiment, different concentrations of paraformaldehyde and glutaraldehyde fixed tissue selection, do CO histochemical staining, and light and electron microscopy and found that 1% paraformaldehyde and 2% glutaraldehyde mixed fixed both can save good tissue The structure can obtain higher CO activity.