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本文采用磷酸缓冲生理盐水(PDS)从门脉灌洗小鼠肝脏,经匀浆管研磨肝组织,制备肝浸液。肝浸液越早加入淋巴细胞培养中,其抑制活性越明显。肝浸液的此种免疫抑制活性在-20℃时可保持70d,在-70℃时,至少可保存190d。推测肝浸液中主要含有一种不被透析、不耐热、被强酸(pH≤4.6)和强碱(pH>11)所破坏,能耐受低温、冻融和能被50～70％饱和硫酸铵盐析的蛋白质。部分纯化的肝脏免疫抑制蛋白,在高浓度时,能强烈抑制淋巴细胞对促有丝分裂原的增生反应,而低浓度时,能增强淋巴细胞增生反应,因而它具有双向作用,即免疫调节作用。此外,肝浸液中可能还含有能与~3H-胸苷(~3H-dT)竞争的核苷类。
In this study, the liver of mice was lavaged from portal vein using phosphate buffered saline (PDS). The liver tissue was ground by homogenization and liver infusion was prepared. The sooner liver infusion is added to lymphocyte culture, the more obvious its inhibitory activity is. The immunosuppressive activity of liver extract can be maintained at -20°C for 70 days and at -70°C for at least 190 days. It is presumed that the liver immersion fluid mainly contains one kind of non-dialysed, heat-resistant, destroyed by strong acid (pH ≤ 4.6) and strong alkali (pH> 11), can tolerate low temperature, freezing and thawing, and can be saturated by 50 to 70%. Ammonium sulfate salted protein. Partially purified hepatic immunosuppressive proteins, at high concentrations, strongly inhibit the proliferative response of lymphocytes to mitogens, while at low concentrations, they enhance lymphocyte proliferation and thus have a bidirectional effect, ie, immunomodulatory effects. In addition, liver extracts may also contain nucleosides that compete with ~3H-thymidine (~3H-dT).