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目的对甲型肝炎减毒活疫苗加强免疫的可能性进行研究。方法分别设置再免疫组、初免疫组和自然感染组。用ＡｂｂｏｔＥＩＡ改良法定性和定量检测血清中的甲型肝炎抗体。结果再免疫后甲型肝炎抗体阳性率和几何平均滴度在２周内升高，与初免疫组抗体阳性率和几何平均滴度缓慢上升至３个月以后达到高峰形成对比。再免疫组和自然感染组对活疫苗免疫反应存在区别，这在研究疫苗免疫持久性时应予考虑。再免疫组初免成功抗体又转阴者，再免疫后抗体阳性率８３３％（１０／１２），显著高于初免疫失败者再免疫后的阳性率５０％（１７／３４），两者又均高于初免疫者（２９６％）。结论再免疫可引起免疫回忆反应，且初免疫成功率直接影响到再免疫的效果。ＡｂｂｏｔＥＩＡ改良法检测血清中甲型肝炎抗体仍低估了原发性免疫反应，需用更为灵敏特异的方法检测疫苗抗体。
Objective To study the possibility of enhancing the immunity of live attenuated hepatitis A vaccine. Methods Re-immunization group, initial immunization group and natural infection group were set up respectively. Hepatitis A antibodies in sera were assayed qualitatively and quantitatively with AbbotEIA. Results After re-immunization, the positive rate of hepatitis A antibody and the geometric mean titer increased in 2 weeks, which was in contrast with the peak of antibody positive rate and geometric mean titer in initial immunization group rising slowly to 3 months later. There is a difference in the immune response between the re-immunized group and the naturally infected group on live vaccines, which should be considered when studying vaccine immunity persistence. In the re-immunization group, the antibody positive rate was 833% (10/12), which was significantly higher than that of the first immunization after immunization (50%, 17/34) Who were higher than the initial immunization (29 6%). Conclusion Re-immunization can cause immune recall reaction, and the success rate of initial immunization directly affects the re-immunization effect. AbbotEIA improved detection of serum hepatitis A antibodies still underestimated the primary immune response, the need for more sensitive and specific detection of vaccine antibodies.