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抗菌素 G-418(Ⅰ)是由红橙小单孢菌(Micro-monospora rhodorangea)产生的一种新的氨基糖甙抗菌素。根据分类学和在各种标准培养基上的生长特征,红橙小单孢菌鉴定为一个新种,与紫色小单孢菌(M.purpurea)最为接近。沉没发酵培养基(克/计)为:黄豆粉30,糊精50,葡萄糖5,碳酸钙7和氯化钻2.4×10~(-4)。接种5%体积的种子后,于35℃培养2～5天。发酵液用草酸处理,调节 pH 至2,过滤,以氧氧化铵中和,经 AmberliteIRC-50(NH_4~+型)树
Antibiotics G-418 (I) is a new aminoglycoside antibiotic produced by Micro-monospora rhodorangea. Micromonospora sp. Was identified as a new species based on taxonomy and growth characteristics on various standard media, most closely to M.purpurea. Sinking fermentation medium (g / meter): soybean flour 30, dextrin 50, glucose 5, calcium carbonate 7 and diamond drill 2.4 × 10 -4. After inoculation of 5% by volume of seeds, they are incubated at 35 ° C for 2 to 5 days. Fermentation broth was treated with oxalic acid, pH was adjusted to 2, filtered, neutralized with ammonium oxalate and purified by Amberlite IRC-50 (NH 4 - + type)