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目的探讨视网膜母细胞瘤相关蛋白46(RbAp46)基因对白血病细胞的作用机制。方法将全长RbAp46基因的cDNA转染人白血病细胞系K562细胞及人脑膜胶质瘤细胞系SHG44细胞, 建立稳定过度表达RbAp46基因的细胞株,观察细胞的生物学行为的同时,通过RT-PCR方法寻找表达差异的相关基因。结果过度表达RbAp46基因的K562细胞及SHG44细胞生长受抑。表现在 K562／RbAp46细胞与K562／CMV细胞于生长第4天分别为(90．00±8．40)×10~4和(119．58±9．87)× 10~4,SHC44／RbAp46细胞与SHG44／CMV细胞于生长第5天分别为(89．13 4±4．88)×10~4和(149．42± 10．83)×10~4。生长第7天时,K562／RbAp46细胞和K562／CMV细胞集落数分别为131．67±15．57和 250．33±26．31(P<0．01),SHG44／RbAp46细胞和SHG44／CMV细胞集落数分别为50．78±6．77和 206．67±37．18(P<0．01)。K562／RbAp46细胞与K562／CMV细胞S期细胞分别占(48．88±4．35)％和(62．78±4．78)％(P<0．01)。G_0／G_1期细胞分别占(29．10±4．14)％和(22．40±2．43)％(P< 0．05),而SHG44／RbAp46细胞与SHG44／CMV细胞G_0／G_1期细胞分别占65．6％和48．8％,S期细胞分别占22．6％和38．4％。过度表达RbAp46基因的K562细胞出现胰岛素样生长因子结合蛋白相关蛋白1(IGFBP-rPl)基因的诱导性表达。结论在K562细胞中可能存在RbAp46和IGFBP-rPl基因之间的调控通路。
Objective To investigate the mechanism of retinoblastoma-associated protein 46 (RbAp46) gene in leukemia cells. Methods Full-length cDNA of RbAp46 gene was transfected into human leukemia cell line K562 and human glioma SHG44 cells to establish a cell line stably overexpressing RbAp46 gene. The biological behavior of RbAp46 gene was observed by RT-PCR Methods to find the expression of differences in the relevant genes. Results The growth of K562 cells and SHG44 cells overexpressing RbAp46 gene were inhibited. The expression of SHC44 / RbAp46 cells in K562 / RbAp46 cells and K562 / CMV cells on day 4 were (90.00 ± 8.40) × 10 ~ 4 and (119.58 ± 9.87) × 10 ~ And SHG44 / CMV cells were (89.13 4 ± 4.88) × 10 ~ 4 and (149.42 ± 10.83) × 10 ~ 4 on the fifth day of growth. On the 7th day of growth, the number of colony of K562 / RbAp46 and K562 / CMV cells were 131.67 ± 15.57 and 250.33 ± 26.31 (P <0.01), respectively. SHG44 / RbAp46 cells and SHG44 / CMV cells The number of colonies was 50.78 ± 6.77 and 206.67 ± 37.18, respectively (P <0.01). The percentage of S phase cells in K562 / RbAp46 cells and K562 / CMV cells were (48.88 ± 4.35)% and (62.78 ± 4.78)%, respectively (P <0.01). G_0 / G1 phase cells accounted for (29.10 ± 4.14)% and (22.40 ± 2.43)%, respectively (P <0.05), while SHG44 / RbAp46 cells and SHG44 / The cells accounted for 65.6% and 48.8% respectively, while S cells accounted for 22.6% and 38.4% respectively. Inducible expression of insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) gene appears in K562 cells overexpressing RbAp46. Conclusion There may be a regulatory pathway between RbAp46 and IGFBP-rP1 in K562 cells.