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定期监测糖尿病患者的尿微量白蛋白,对尚处于可逆性阶段糖尿病性肾病的早期诊断具有重要价值。尿白蛋白也是高血压病引起血管损伤及肾血管早期病变的敏感指标。我们自制高效价抗人Alb血清、抗(人Alb)IgG-HRP酶标记结合物和抗(人Alb)IgG包被微孔滴定板,建立了一种灵敏、简便的尿微量白蛋白酶联免疫测定法,测定范围为5～500ng/ml;灵敏度0.76ng;批内CV％ 6.4％;批间CV％ 16.4％。24小时尿白蛋白排泌量,正常人为8.86(SD 9.23)mg/24h(n=80);糖尿病患者为40.4(SD 40.4)mg/24h(n=16);Ⅰ、Ⅱ、Ⅲ期高血压病患者分别为15.17(SD 14.42)mg/24h、21.46(SD 24.34)mg/24h和24.6(SD 18.9)mg/24h。
The regular monitoring of urinary microalbuminuria in diabetic patients is of great value in the early diagnosis of diabetic nephropathy which is still in the reversible phase. Urinary albumin is also a sensitive indicator of vascular damage caused by hypertension and early renal vascular disease. We developed a sensitive and simple urinary microalbumin immunoassay for the production of high titer human Alb serum, anti (human Alb) IgG-HRP enzyme conjugate and anti (human Alb) IgG coated microtiter plate Method, the determination of the range of 5 ~ 500ng / ml; sensitivity 0.76ng; intra-CV% 6.4%; inter-CV% 16.4%. 24 hours urinary albumin excretion, normal 8.86 (SD 9.23) mg / 24h (n = 80); diabetic patients 40.4 (SD 40.4) mg / 24h (n = 16); Ⅰ, Ⅱ, Patients were 15.17 (SD 14.42) mg / 24h, 21.46 (SD 24.34) mg / 24h and 24.6 (SD 18.9) mg / 24h, respectively.