论文部分内容阅读
目的探讨胆管癌组织中6-氧-甲基鸟嘌呤-DNA甲基转移酶(MGMT)基因的甲基化状态及其在胆管癌中的临床意义。方法采用甲基化特异性PCR(MSP)和免疫组织化学法分析胆管癌组织中MGMT基因启动子甲基化状态及MGMT蛋白表达情况,并分析MGMT基因甲基化状态与临床病理特征的关系。结果 36例胆管癌组织中MGMT基因17例(47.2%)为甲基化阳性;MGMT蛋白阳性表达15例,阴性表达21例,21例MGMT蛋白阴性表达的胆管癌中MGMT甲基化阳性14例;15例MGMT蛋白阳性表达中MGMT甲基化阳性仅3例。MGMT蛋白表达与MGMT甲基化状态呈负相关(rs=-0.816,P<0.05)。MGMT基因的甲基化状态与胆管癌患者的肿瘤浸润深度、分化程度及TNM分期有关(P<0.05),但与患者的年龄、性别、病理学类型、淋巴结转移均无关(P>0.05)。结论 MGMT基因启动子过甲基化是胆管癌组织中常见的分子事件,并可能与胆管癌发生有关,MGMT甲基化状态可以作为评价胆管癌恶性程度的一项指标。
Objective To investigate the methylation status of 6-O-methylguanine-DNA methyltransferase (MGMT) gene in cholangiocarcinoma and its clinical significance in cholangiocarcinoma. Methods The methylation status and MGMT protein expression of MGMT gene in cholangiocarcinoma were analyzed by methylation-specific PCR (MSP) and immunohistochemistry. The relationship between the methylation status of MGMT gene and clinicopathological features was analyzed. Results The methylation of MGMT gene in 17 cases (47.2%) was detected in 36 cases of cholangiocarcinoma. The positive expression of MGMT protein in 15 cases was negative in 21 cases. The methylation of MGMT was found in 21 cases of MGMT negative cholangiocarcinoma (14 cases) MGMT methylation was positive in only 15 cases of MGMT protein positive expression in 3 cases. MGMT protein expression and MGMT methylation status was negatively correlated (rs = -0.816, P <0.05). The methylation status of MGMT gene was related to the depth of tumor invasion, differentiation and TNM stage in patients with cholangiocarcinoma (P <0.05), but not with age, gender, pathological type and lymph node metastasis (P> 0.05). Conclusion MGMT gene promoter hypermethylation is a common molecular event in cholangiocarcinoma and may be related to the occurrence of cholangiocarcinoma. MGMT methylation status can be used as an indicator to evaluate the malignancy of cholangiocarcinoma.