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研究了新型环烷基磺酰胺类化合物N-(2,4,5-三氯苯基)-2-氧代环己烷基磺酰胺(简称化合物108)对灰葡萄孢菌丝生长、孢子形成和萌发以及菌核产生等不同生育阶段的抑制作用及其对菌丝致病力和形态结构的影响。结果表明:化合物108对灰葡萄孢菌丝生长和孢子形成及孢子萌发具有明显的抑制作用,其EC50值分别为6.90、4.70和4.11μg/mL;菌核形成受到明显抑制,当药剂质量浓度达20μg/mL时,无菌核产生。经化合物108处理后的灰葡萄孢菌丝致病力下降,40μg/mL处理的菌丝致病力显著低于对照。超微结构观察结果表明,化合物108能导致灰葡萄孢菌丝萎缩、塌陷和变形,菌体细胞壁增厚、皱缩及分层。
The effects of the novel cycloalkylsulfonamides, N- (2,4,5-trichlorophenyl) -2-oxocyclohexanesulfonamide (compound 108) on the growth of C.bicolor spores, sporulation And germination and sclerotia at different stages of growth and its inhibitory effect on mycelial pathogenicity and morphological structure. The results showed that compound 108 had significant inhibitory effect on the growth, spore formation and spore germination of Botrytis cinerea, with EC50 values of 6.90, 4.70 and 4.11 μg / mL, respectively. The formation of sclerotia was significantly inhibited. When the drug concentration reached At 20 μg / mL, aseptic nuclei develop. The pathogenicity of Botrytis cinerea decreased after treatment with compound 108, and the pathogenicity of mycelia treated with 40μg / mL was significantly lower than that of the control. The results of the ultrastructure observation showed that compound 108 could cause the atrophy, collapse and deformation of the mycelium of Botrytis cinerea, thickening, collapsing and delamination of the cell wall of the cell body.