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Objective: To study the effect of glucocorticoid on the promoter of the pre-receptor glucocorticoid metabo-lizing enzyme 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1)gene. Methods: The 1.2 kb length sequenceupstream to the transcription start site of the 11β-HSD1 gene was amplified with polymerase chain reaction (PCR) andthen was cloned into pBLCAT6 plasmid carrying chloramphenicol acetyltransferase (CAT) reporter gene. The plasmidpBLCAT6 carrying the promoter and reporter gene was used to transfect HeLa cells to study the regulation of 11β-HSD1gene expression by glucocorticoids in terms of reporter gene expression. Results: PCR showed that there was a com-plete alignment of the amplified sequence with the sequence 1.2 kb upstream to the transcription start site of 11β-HSD1gene. When cloned into pBLCAT6 plasmid carrying the reporter gene, this part of the promoter is functional in terms ofregulation of reporter gene expression upon transfection into HeLa cells. The synthetic glucocorticoid-dexa
Objective: To study the effect of glucocorticoid on the promoter of the pre-receptor glucocorticoid metabo-lizing enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) gene. Methods: The 1.2 kb length sequence upstream to the transcription start site of the 11β- HSD1 gene was amplified with polymerase chain reaction (PCR) and was cloned into pBLCAT6 plasmid carrying chloramphenicol acetyltransferase (CAT) reporter gene. The plasmidpBLCAT6 carrying the promoter and reporter gene was used to transfect HeLa cells to study the regulation of 11β-HSD1 gene expression by glucocorticoids in terms of reporter gene expression. Results: PCR showed that there was a com-plete alignment of the amplified sequence with the sequence 1.2 kb upstream to the transcription start site of 11β-HSD1 gene. When cloned into pBLCAT6 plasmid carrying the reporter gene, this part of the promoter is functional in terms ofregulation of reporter gene expression upon transfection into HeLa cells. The synthetic g lucocorticoid-dexa