目的了解济宁市医疗卫生机构实验室生物安全现状,为进一步加强和规范辖区医疗卫生机构实验室生物安全管理工作提供依据。方法设计《济宁市医疗卫生机构实验室生物安全调查表》,于2009年6月对辖区35家医疗卫生机构进行问卷调查,数据核查无误后进行统计学处理分析。结果有效问卷回收率100%。各医疗卫生机构均有主管领导负责实验室安全管理;91.4%实验室制定实验室生物安全、实验室消毒及医疗、检验废弃物处理等管理制度;82.9%的单位能做到实验区与办公区分开;80%以上的实验室配备有Ⅱ级生物安全柜、高压蒸汽灭菌器等实验设施;50.0%的实验室操作人员经过培训考核获得上岗证;91.4%的实验室制定了应急操作规范;85.7%的实验室建立了意外事故处理程序;20.0%的实验室贴有生物危害警告标志。结论调查结果显示辖区各级医疗卫生机构在实验室生物安全管理、制度建设、工作人员安全技术培训、安全防护设施设备配备方面,存在不同差距,有待今后进一步完善和加强管理。 Objective To understand the status quo of laboratory biosafety in medical and health institutions in Jining and provide evidence for further strengthening and standardizing biosafety management in laboratories of medical and health institutions in jurisdictions. Methods Design the “Jining Medical and Health Institution Laboratory Biosafety Questionnaire”. In June 2009, a questionnaire survey was conducted on 35 medical and health institutions in the area. Data were verified and statistically analyzed. Results valid questionnaire recovery rate of 100%. 91.4% of the laboratories have established management systems of laboratory biosafety, laboratory disinfection and medical treatment, waste disposal inspection, etc., and 82.9% of the units can do the experiment and office division Open; 80% of the laboratories equipped with biological safety cabinets Ⅱ level, high pressure steam sterilizer and other experimental facilities; 50.0% of laboratory operators to obtain a post examination certificate; 91.4% of laboratories developed emergency operating practices; Accident handling procedures were established in 85.7% of laboratories and biohazard warning signs in 20.0% of laboratories. Conclusions The survey results show that there are differences in the management of biosafety, establishment of systems, technical training of safety staff and equipment of safety protection facilities in all levels of medical and health institutions at the prefecture level, awaiting further improvement and strengthening of management in the future.