1、取材及接种取葡萄幼嫩新梢端部20厘米长的枝段,去掉叶片,保留两节以上。先用清水冲洗干净,然后用饱和漂白粉溶液消毒15—20分钟或用0.1%氯化汞溶液消毒5—10分钟,在接种箱内,用无菌水冲洗3、4次,用无菌滤纸吸去多余的水分.剪去两端被消毒药物损伤的部分,再剪成2—3厘米长的单芽茎段并使芽位于茎段的上端。然后,插入事先准备好的培养基中,扦插深度以芽眼露出培养基表面3—4毫米为宜.材料接种后在培养室培养,培养室的温 1, drawn and inoculated grapes take young tip 20 cm long branches, remove the leaves, to retain more than two. Rinse with clean water first and then sterilize with saturated bleach solution for 15-20 minutes or disinfect with 0.1% mercuric chloride solution for 5-10 minutes. Flush with sterile water for 3 to 4 times in inoculation box, Remove excess water, cut off the damaged parts of both ends of the disinfectant, and cut into 2-3 cm long single shoots and the buds at the top of the stems. Then, insert the medium prepared in advance, the depth of cuttings bud surface exposing the surface of the medium 3-4 mm is appropriate material after inoculation culture room culture room temperature