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目的观察灯盏花素对谷氨酸致原代培养的大鼠脑微血管内皮细胞(rBMECs)损伤的保护作用。方法灯盏花素(200、100、50μmol/L)作用于rBMECs 24 h后,加入谷氨酸(终浓度为1 mmol/L)培养18 h,MTT法检测rBMECs细胞活性,并按试剂盒方法检测细胞上清液中乳酸脱氢酶(LDH)水平、细胞中丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性。结果谷氨酸(l mmol/L)使原代培养的rBMECs明显受到损伤,灯盏花素高、中浓度(200、100μmol/L)可显著对抗谷氨酸造成的rBMECs损伤,抑制LDH释放,降低MDA水平,增强SOD活性。结论灯盏花素对谷氨酸所致原代培养的rBMECs损伤具有明显的保护作用,其机制与增强细胞抗氧化能力有关。
Objective To observe the protective effect of breviscapin on the injury of primary cultured rat brain microvascular endothelial cells (rBMECs) induced by glutamic acid. Methods Breviscapine (200, 100 and 50 μmol / L) was used to effect rBMECs for 24 h, then cultured for 18 h with addition of glutamic acid (final concentration was 1 mmol / L). The cell viability was measured by MTT assay and detected by kit Lactate dehydrogenase (LDH) level, MDA level and superoxide dismutase (SOD) activity in the cell supernatant. Results Glutamic acid (1 mmol / L) significantly damaged primary cultured rBMECs. Breviscapine high and medium concentrations (200 and 100 μmol / L) significantly inhibited glutamate-induced rBMECs injury, inhibited LDH release and decreased MDA level, enhance SOD activity. Conclusion Breviscapine has a significant protective effect on primary cultured rBMECs induced by glutamate, and its mechanism is related to its ability to enhance antioxidant capacity.