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目的缩短红杆菌(Rubrobacter sp.)YIM93620和台湾红杆菌(Rubrobacter taiwanensis)BCRC17173培养时间,加快试验进度,更方便研究其耐辐射水平;确定这两株菌对重离子照射后的生物学效应,为航天辐射防护提供理论支持。方法比较这两株菌在TSB、CYC、Thermus、ISP2这四种培养基上的生长情况,并测定添加不同浓度的丙酮酸钠和甜菜碱对生长的促进情况;选E.coli K-12作为阴性对照,分别对这两株菌进行不同剂量的~(12)C~(6+)重离子照射,并绘制致死率曲线研究生物学效应。结果 YIM93620和BCRC17173在Thermus培养基中生长状态最好;分别在添加0.125%甜菜碱和0.1%丙酮酸钠的Thermus培养基中培养时间从10~15 d缩短到6 d;YIM93620在160 Gy时达到最高致死率93.3%,而BCRC17173在320 Gy时致死率仅35.7%,对~(12)C~(6+)重离子都具有强的抗性。结论筛选到这2株菌生长状态都较好的培养基Thermus,添加0.125%甜菜碱和0.1%丙酮酸钠分别可显著促进菌株YIM93620和BCRC17173的生长;这两株菌均有强的耐~(12)C~(6+)重离子辐射特性。
OBJECTIVE To shorten the culture time of Rubrobacter sp. YIM93620 and Rubrobacter taiwanensis BCRC17173, to speed up the test progress and to study the radiation resistance of the two strains more easily. To determine the biological effects of these two strains on heavy ion irradiation, Space radiation protection to provide theoretical support. Methods The growth of TSB, CYC, Thermus and ISP2 was compared between the two strains. The growth of TSB, CYC, Thermus and ISP2 were also compared. The growth of TSB, Negative control, respectively, the two strains of different doses of ~ (12) C ~ (6 +) heavy ion irradiation, and draw the lethality curve to study the biological effects. Results YIM93620 and BCRC17173 had the best growth in Thermus medium. The incubation time in Thermus medium supplemented with 0.125% betaine and 0.1% sodium pyruvate was shortened from 10 to 15 days to 6 days respectively. The YIM93620 reached at 160 Gy The highest lethality was 93.3%. However, the lethality of BCRC17173 was only 35.7% at 320 Gy and strong resistance to ~ (12) C ~ (6 +) heavy ions. Conclusion The results showed that the growth of these two strains was better than that of Thermus. The addition of 0.125% betaine and 0.1% sodium pyruvate significantly promoted the growth of strain YIM93620 and BCRC17173, respectively. 12) C ~ (6 +) heavy ion radiation characteristics.