马铃薯是世界上最重要的粮食作物之一，但其生产却受到病害的严重限制．本文简介了马铃薯抗病育种新途径一生物技术的应用及其研究进展，主要包括：茎尖脱毒，离体试管薯诱导，花药和花粉培养，胚挽救，抗病突变体离体筛选，原生质体融合技术和农杆菌介导的外源基因转化．这些技术与常规育种手段相结合已取得具有应用价值的结果． Potato is one of the most important food crops in the world, but its production is severely limited by diseases. In this paper, we introduced the application and research progress of a new biotech technology for potato disease-resistant breeding, including: tips of virus-free shoot tips, induction of in vitro sweet potato, anther and pollen culture, embryo rescue, screening of resistant mutant in vitro, Body Fusion and Agrobacterium - mediated Transformation of Foreign Genes. These technologies, combined with conventional breeding techniques, have yielded results of application value.