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目的:考察2种新型金属卟啉化合物(药物-A:四水合氯化-氯-二-2,2’-联吡啶(-5-吡啶基-10,15,20-三苯基卟啉)合钌(Ⅱ)(Ru(bpy)2TMPP)C12);药物-B:四水合氯化-氯-二-菲咯啉(-5-吡啶基-10,15,20-三苯基卟啉)合钌(Ⅱ)(Ru(phen)2TMPP)C12)),对人宫颈癌Hela细胞株的体外抗癌活性。方法:采用MTT法测定药物对Hela细胞增殖的影响;邻二氮菲-Fe2+氧化法测定药物对细胞清除羟自由基能力的影响;Giemsa染色法观察细胞形态学的改变。结果:药物-A、B抑制Hela细胞的最适宜条件为1×10-5mol/L培养48h并光照2h。随药物浓度升高肿瘤细胞的羟自由基清除能力相应下降,给光比避光作用更明显;Giemsa染色后光学显微镜下观察到给药后的细胞形态数目发生不同程度的变化,在最高浓度处可观察到大量细胞脱壁和细胞碎片浮起,凋亡细胞数量明显增多。结论:2种药物对Hela细胞均有杀伤作用,作用的强弱与给药的浓度和光照时间有关。
OBJECTIVE: To investigate the effects of two new metalloporphyrins (drug-A: chlorinated tetrachloro-chloro-bis-2,2’-bipyridyl) -5-pyridyl-10,15,20-triphenylporphyrin Ruthenium (II) (Ru (bpy) 2TMPP) C12); drug-B: chlorinated bischlorophenanthroline tetrahydropyridine (5-pyridyl-10,15,20-triphenylporphyrin) Ruthenium (Ⅱ) (Ru (phen) 2TMPP) C12) on human cervical cancer Hela cell line in vitro anticancer activity. Methods: The effects of drugs on the proliferation of Hela cells were determined by MTT method. The effects of drugs on the scavenging of hydroxyl free radical were measured by phenanthroline-Fe2 + oxidation method. The morphological changes were observed by Giemsa staining. Results: The most suitable conditions for inhibiting Hela cells by drug-A and B were 1 × 10-5mol / L for 48h and light for 2h. With the increase of drug concentration, the scavenging capacity of hydroxyl radical of tumor cells decreased correspondingly, and the effect of light on the darkness was more obvious. Under the light microscope after Giemsa staining, the number of cell morphology changed to some extent after administration, A large number of cell detachment and cell debris floating were observed, and the number of apoptotic cells increased significantly. Conclusion: The two drugs have killing effect on Hela cells, and the intensity of action depends on the concentration and illumination time.