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目的:采用不同NO_3~-:NH_4~+比的营养液,探索不同氮源对钼元素在两种甘蓝型油菜(L0917和ZS11)中的吸收、转运和分布的影响及品种间的差异。创新点:在不同氮源条件下,从组织分布、汁液运输、亚细胞分布以及品种等方面研究了甘蓝型油菜的钼营养状况。方法:将甘蓝型油菜L0917和ZS11的幼苗在正常营养液培养20天后,分别转移至4种NO_3~-:NH_4~+比(14:1、9:6、7.5:7.5、1:14)且总氮为15 mmol/L的营养液中培养15天后收获。采用原子吸收分光光度计-石墨炉法测定根、茎和叶不同部位钼含量,木质部和韧皮部液钼含量,叶肉细胞细胞壁组分、细胞器组分和可溶性组分钼含量。结论:在高或低NO_3~-:NH_4~+比条件下,高的钼吸收能力、木质部转运、韧皮部再迁移以及叶片亚细胞钼储存在甘蓝型油菜钼积累上扮演重要角色。
OBJECTIVE: To investigate the effects of different nitrogen sources on the uptake, transport and distribution of molybdenum in two types of Brassica napus (L0917 and ZS11) and to explore the differences among the cultivars by using nutrient solutions with different NO_3 ~ -: NH_4 + ratios. Innovative point: Under different nitrogen sources, the status of molybdenum nutrition in Brassica napus was studied from the aspects of tissue distribution, juice transportation, subcellular distribution and variety. Methods: Seedlings of L0917 and ZS11 in Brassica napus were transferred to four kinds of NO_3 ~ -: NH_4 ~ + (14: 1, 9: 6, 7.5: 7.5, 1:14) Total nitrogen 15 mmol / L of nutrient solution after 15 days of harvest. The content of molybdenum, the content of molybdenum in xylem and phloem, the cell wall component of mesophyll cell, the organelle component and the content of soluble component molybdenum in different parts of root, stem and leaf were measured by atomic absorption spectrophotometer-graphite furnace method. CONCLUSION: High molybdenum absorption capacity, xylem transport, phloem re-migration, and leaf subcellular molybdenum storage play an important role in the accumulation of molybdenum in Brassica napus under high or low NO_3 ~ -: NH_4 ~ + conditions.