本文列举了原有根结线虫会阴花纹扫描电镜样品制备法存在的问题,叙述了改进后的程序。与原法比较,该程序具有费时少,操作简便,效果好等优点。植物根结线虫病害在我国发生普遍,寄主范围广,在林木上可为害多种树木,尤以苗期受害最为严重,受害较重的树种有泡桐、法国梧桐、油橄榄、梓树、楸树、桑树、象耳豆等。应用扫描电镜观察根结线虫会阴花纹,揭示虫体会阴部角质膜的复杂细微结陶,是线虫分类上的一种极有价值的方法,但目前,在扫描电镜制样过程中,还存 This article lists the existing root knot nematode perineal pattern scanning electron microscopy sample preparation problems, describes the improved program. Compared with the original method, the program has the advantages of less time-consuming, simple and effective. Plant root-knot nematode disease is common in our country, with a wide range of hosts, many kinds of trees can be harmed on the trees, especially the most severely damaged seedling trees are paulownia, sycamore, olive, catalpa, catalpa, Mulberry, like ear beans and so on. Scanning electron microscopy to observe the root knot nematode perineal pattern reveals the perineal perineal complex delicate knot Tao Jie, is a nematode classification of a valuable method, but at present, the scanning electron microscopy sample preparation process,