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目的了解合肥地区慢性无症状乙肝病毒携带者(As C)体内HBV s基因的变异情况。方法自慢性肝炎患者血清中提取HBV基因组DNA,扩增其s基因片段并进行序列分析,用MEGA 3.1和Clustalx 1.8软件为所测基因进行分型,并通过比对s基因序列,分析s基因变异情况。结果 27例样品中,B型13例(48.1%),C型14例(51.9%),未发现混合型和其他基因型。有12例(44.4%)标本s基因发生突变,其中B型6例,C型6例;单位点突变有8例,多位点突变有4例,其中B型有2例在氨基酸133位点发生突变(Met→Leu)。结论 27例慢性无症状乙肝病毒携带者HBV的基因型为B型和C型,As C患者体内HBV病毒s基因存在突变现象,并且突变位点随机,突变形式有单点突变和多位点突变。
Objective To investigate the variation of HBV s gene in chronic asymptomatic hepatitis B virus carriers (As C) in Hefei area. Methods The HBV genomic DNA was extracted from the serum of patients with chronic hepatitis and its s gene fragment was amplified and sequenced. The genotypes were determined by MEGA 3.1 and Clustalx 1.8 software. Happening. Results Of the 27 samples, 13 were type B (48.1%) and 14 (51.9%) were type C, no mixed and other genotypes were found. There were 12 cases (44.4%) of the s gene mutations, including 6 cases of type B, 6 cases of type C; 8 cases of single point mutation, multi-site mutation in 4 cases, including 2 cases of type B in the amino acid 133 sites A mutation occurs (Met → Leu). Conclusion The genotypes of HBV in 27 patients with chronic asymptomatic hepatitis B virus are type B and C. The mutation of HBV s gene in As C patients is mutated, and the mutation sites are random and the mutation forms are single point mutations and multiple site mutations .