论文部分内容阅读
用带有254nm紫外检测器的高效液相色谱仪,反相色谱法测定血清中阿米替林及其代谢产物去甲替林,E-10-OH-阿米替林,E-10-OH-去甲替林和N-去甲基去甲替林浓度。方法为碱化血清,以乙醚提取后挥干,残留物溶解后注入国产色谱柱(YWG-C_(18)) 。用盐酸赛庚啶做内标。各测定物的日内回收率为89.2-110％,变异系数小于8.8％。日间回收率为95.1-111％,变异系数小于9.6％。最低检测限为2ng,血清中最低有效测定浓度为20ng/ml。本法适用于科研或临床监测血药浓度。
Determination of amitriptyline and its metabolites nortritin, E-10-OH-amitriptyline, E-10-OH in serum by high performance liquid chromatography with 254 nm UV detector - nortriptyline and N-desmethyl nortriptyline concentrations. The method is basified serum, extracted with ether and evaporated to dryness, the residue dissolved into the domestic column (YWG-C_ (18)). Use cyproheptadine as internal standard. The daily recovery of each assay was 89.2-110% and the coefficient of variation was less than 8.8%. Daytime recovery was 95.1-111% with a coefficient of variation of less than 9.6%. The minimum detectable limit is 2 ng and the lowest effective concentration in serum is 20 ng / ml. This law applies to scientific research or clinical monitoring of plasma concentrations.