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为明确齐齐哈尔市玉米穗腐病病原菌,于2013年自黑龙江省齐齐哈尔市玉米种植区采集玉米穗腐病样品,进行病原物分离、鉴定及致病性测定研究。结果表明:分离的菌株菌落形态、分生孢子形态与禾谷镰孢菌相同,但不分泌色素;ITS基因序列与Genebank登记中的禾谷镰孢菌HQ671191.1同源性为100%;系统发育树状图结果显示该菌株与禾谷镰孢菌位于系统发育树的同一分支;按照科赫氏法则进行致病性测定,证实分离的病原菌可造成严重的玉米果穗腐烂。因此将该菌株命名为禾谷镰孢菌齐齐哈尔变种。
In order to clarify the pathogen of corn ear rot in Qiqihar City, corn ear rot samples were collected from corn growing area of Qiqihar in Heilongjiang Province in 2013, and the pathogen isolation, identification and pathogenicity determination were studied. The results showed that the colony morphology and conidial morphology of the isolates were the same as those of Fusarium graminearum, but did not secrete pigment. The homology of ITS gene sequence to Fusarium graminearum HQ671191.1 in Genebank was 100% The developmental dendrogram showed that the strain was located on the same branch of Fusarium graminearum as the phylogenetic tree. The pathogenicity test was conducted according to the Koch’s rule, which confirmed that the isolated pathogen could cause severe ear rot in maize. Therefore, the strain named Fusarium graminearum Qiqihar variant.