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目的对自然妊娠与辅助生殖技术(ART)术后妊娠胚胎停育患者绒毛细胞染色体进行研究,分析自然妊娠与ART术后妊娠绒毛细胞染色体异常率,并通过二者之间的比较,从遗传学角度来验证ART手术是否安全。方法通过搜集患者绒毛组织,按照常规方法制备染色体涂片,通过G显带法进行染色体核型分析,并应用SPSS 13.0软件进行统计分析,比较2组间绒毛细胞染色体异常率的差异。结果异常核型共计145例,异常核型检出率为56.20%(145/258),以常染色体三倍体为主。自然流产绒毛细胞正常核型为63例,异常核型为73例,异常率为53.67%(73/136)。ART组正常核型为50例,异常核型为72例,异常率为59.01%(72/122)。自然流产组与ART组绒毛染色体异常率比较,差异无统计学意义(P>0.05)。结论早期胚胎停育与胚胎染色体异常密切相关,自然妊娠与ART术后妊娠绒毛细胞染色体异常率无显著差异。
OBJECTIVE: To study the chromosomal aberrations of chorionic villi in gestational febrile pregnancy after natural pregnancy and assisted reproductive technology (ART), and to analyze the chromosomal abnormalities in chorionic villi between natural pregnancy and ART after the operation. Through the comparison between the two methods, To verify the safety of ART surgery. Methods Chromosome smears were collected by conventional methods and chromosome karyotypes were analyzed by G banding method. SPSS 13.0 software was used for statistical analysis. The differences in chromosomal abnormalities between two groups were compared. Results A total of 145 cases of abnormal karyotype, abnormal karyotype detection rate was 56.20% (145/258), with autosome triploid based. Natural abortion hair cells normal karyotype was 63 cases, 73 cases of abnormal karyotype, the abnormal rate was 53.67% (73/136). The normal karyotype in ART group was 50 cases and the abnormal karyotype was 72 cases, the abnormality rate was 59.01% (72/122). There was no significant difference in chromosomal abnormalities between spontaneous abortion group and ART group (P> 0.05). Conclusion Early embryo suspension is closely related to chromosomal abnormalities in embryos. There is no significant difference in chromosome abnormalities between gestational females and normal controls.