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目的:探讨复心煎对大鼠心肌缺血再灌注损伤保护作用的机制。方法:实验大鼠随机分为伪手术组、模型组、氟伐他汀组(4 mg·kg-1)及复心煎高、低剂量组(按生药量计为28,14 g·kg-1),预先ig给药1周后,采用结扎冠状动脉法制备心肌缺血再灌注损伤模型,最后处死大鼠,HE染色观察大鼠心肌组织病理形态学变化,检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及一氧化氮(NO)的含量,TUNEL法测定心肌细胞的凋亡,RT-PCR测定心肌组织诱导型一氧化氮合酶iNOS mRNA的表达。结果:复心煎高、低剂量组能明显改善缺血再灌注所致心肌组织病理损害;复心煎高剂量组MDA含量(4.49±0.42)μmol·L-1,与模型组(5.27±0.69)μmol·L-1比较明显降低(P<0.05);高剂量组SOD和GSH-Px活性分别为(127.64±19.54)U·mL-1、(534.10±40.82)μmol·L-1,与模型组(104.58±20.54)U·mL-1、(483.85±33.29)μmol·L-1比较明显升高(P<0.05);复心煎低剂量组与模型组相比,各血清指标无显著性差异;复心煎高、低剂量组凋亡指数分别为(19.11±5.78)%,(24.63±4.10)%,与模型组(29.94±6.77)%比较明显降低(P<0.01,P<0.05);复心煎能一定程度上抑制iNOS mRNA的表达。结论:复心煎对大鼠心肌缺血再灌注损伤具有保护作用,其机制可能与清除氧自由基,抑制心肌细胞凋亡和降低iNOS mRNA表达有关。
Objective: To investigate the protective effect of Fuxin Jianpi on myocardial ischemia-reperfusion injury in rats. Methods: The rats were randomly divided into sham-operation group, model group, fluvastatin group (4 mg · kg-1) and high-dose and low-dose group ), Pre-ig administration for 1 week, the model of myocardial ischemia-reperfusion injury was established by ligating the coronary artery. Finally, the rats were sacrificed and the pathological changes of myocardial tissue were observed by HE staining. The levels of superoxide dismutase (SOD) ), Malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and nitric oxide (NO) were measured. The apoptosis of cardiomyocytes was detected by TUNEL method. Nitric oxide synthase iNOS mRNA expression. Results: Compared with the model group (5.27 ± 0.69), the MDA level in the high-dose Fuxin decoction group (4.49 ± 0.42) μmol·L-1 and the low dose group (P <0.05). The activities of SOD and GSH-Px in high dose group were (127.64 ± 19.54) U · mL-1, (534.10 ± 40.82) μmol·L-1, (104.58 ± 20.54) U · mL-1 and (483.85 ± 33.29) μmol·L-1, respectively. Compared with the model group, there was no significant difference in serum indexes (19.11 ± 5.78)% and (24.63 ± 4.10)%, respectively, which were significantly lower than those in the model group (29.94 ± 6.77)% (P <0.01, P <0.05) Fuxin decoction can inhibit the expression of iNOS mRNA to a certain extent. Conclusion: Fuxin decoction has a protective effect on myocardial ischemia-reperfusion injury in rats. The mechanism may be related to scavenging oxygen free radicals, inhibiting cardiomyocyte apoptosis and decreasing iNOS mRNA expression.