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目的:通过动物实验观察蓬子菜水煎液对动脉粥样硬化模型大鼠的影响,从抗炎及对NF-κB信号通路的影响等角度探讨蓬子菜水煎液对动脉硬化的作用机制。方法:通过复合方法建立了动脉粥样硬化模型大鼠。采用蓬子菜水煎液不同剂量灌胃治疗,通塞脉片灌胃作对照,应用免疫组化方法检测病变组织中NF-κB(P65)的表达;应用ELISA法检测ICAM-1、VCAM-1、P-seletin含量。结果:蓬子菜水煎液高中低剂量组NF-κB(P65)的水平与病理模型组及通塞脉片组相比有显著性差异(P<0.05)。蓬子菜水煎液组及通塞脉片组大鼠血清中ICAM-1、VCAM-1、P-seletin的表达与病理模型组相比显著下降,有显著性差异(P<0.05)。蓬子菜水煎液高剂量组与通塞脉片组有显著性差异(P<0.05)。结论:蓬子菜水煎液可降低NF-κB蛋白在病变组织中的表达,降低AS大鼠血清中ICAM-1、VCAM-1、P-seletin的水平,且优于通塞脉片组。
OBJECTIVE: To observe the effects of Phragmites australis Decoction on rats with atherosclerosis through animal experiments and to explore the mechanism of action of Phragmites australis Decoction on arteriosclerosis from the aspects of anti-inflammation and its influence on NF-κB signaling pathway . Methods: Atherosclerosis model rats were established by compound method. The rats were treated with different dosages of Pholiotae decoction and intragastric administration of Tongsaimailian tablet. Immunohistochemistry was used to detect the expression of NF-κB (P65) in the diseased tissues. The expressions of ICAM-1 and VCAM- 1, P-seletin content. Results: The levels of NF-κB (P65) in the low, medium and high doses of Phragmites australis decoction group were significantly different from those in the pathological model group and Tongsaimailian group (P <0.05). The expressions of ICAM-1, VCAM-1 and P-seletin in the pancreas decoction group and Tongsaimailian group were significantly decreased compared with the pathological model group (P <0.05). There was a significant difference (P <0.05) between the high-dose group and the Tongsianmaipian group. Conclusion: The decoction of Phragmites australis can reduce the expression of NF-κB in the diseased tissue and decrease the level of ICAM-1, VCAM-1 and P-seletin in the serum of AS rats, which is better than that of Tongsaimailian tablet group.