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目的研究基底前脑ＮＯＳ神经元移植至成年鼠海马内后，ＮＯＳ的发育变化规律，同时观察移植物与宿主海马间发生联系的情况。方法将大鼠１４～１６ｄ胚胎的基底前脑移植到单侧穹隆海马伞切断的成年大鼠海马内，动物在移植后存活５、７、１４、３０、６０、９０、１５０和１８０ｄ分别取脑，经ＮＡＤＰＨ－ｄ法和尼氏染色观察。结果在移植后第７ｄ时ＮＡＤＰＨ－ｄ阳性染色才出现在ＮＯＳ阳性神经元内，随着移植物成活时间的延长，ＮＯＳ阳性神经元逐渐生长在３０ｄ时已发育成熟，而且３０ｄ时可见其与宿主海马发生联系，直到１８０ｄ仍有良好的联系。结论移植物的ＮＯＳ阳性神经元在海马的发育有一定的规律。移植物可以在海马内长期存活并可以和宿主脑整合生长，表明移植物可能有功能。
Objective To study the development and changes of NOS in basal forebrain NOS neurons transplanted into hippocampus of adult rats and to observe the relationship between the graft and host hippocampus. Methods The basal forebrain of 14 ~ 16 d embryos was transplanted into the hippocampus of adult rats cut off by unilateral vault hippocampus. The animals survived for 5, 7, 14, 30, 60, 90, 150 and 180 days after transplantation. , By NADPH-d method and Nissl staining observation. Results NADPH-d positive staining appeared in NOS positive neurons on the 7th day after transplantation. As the survival time of the grafts increased, NOS positive neurons gradually developed at 30 days and matured at 30 days, Hippocampus contact, until 180d still have good contact. Conclusion The NOS positive neurons in the graft have some regularities in the development of the hippocampus. Grafts can survive long-term in the hippocampus and integrate with the host brain, suggesting that the graft may function.