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目的:观察血腑逐瘀胶囊对大鼠缺血心肌细胞Sirt1信号通路的干预作用,探讨该中药复方的作用机制。方法:将70只健康成年雌性Wistar大鼠随机分成正常组,假手术组(以蒸馏水10 m L·kg-1灌胃),模型组(以蒸馏水10 m L·kg-1灌胃),血腑逐瘀高、中、低剂量组(0.03,0.02,0.01 g·kg-1),L-NAME组(按2 mg/只腹腔注射),采用实时荧光定量PCR(Realtime PCR)技术检测各组心肌组织沉默信息调节因子1(Sirt1),p53及核转录因子-κB(NF-κB)mRNA表达情况。结果:与假手术组比较,模型组缺血心肌细胞Sirt1 mRNA表达水平明显降低(P<0.05),缺血心肌细胞p53,NF-κB mRNA表达水平增高(P<0.05);与模型组比较,血腑逐瘀高、中、低剂量组大鼠缺血心肌细胞Sirt1 mRNA表达水平明显增高(P<0.05),血腑逐瘀高剂量组大鼠缺血心肌细胞p53,NF-κB mRNA表达水平明显降低(P<0.05)。结论:血腑逐瘀胶囊可延长缺血心肌细胞寿命,其机制可能是通过激活Sirt1信号通路,抑制p53,NF-κB基因的表达而发挥作用。
Objective: To observe the intervention of Xuefuzhuyu capsule on Sirt1 signaling pathway in ischemic cardiomyocytes in rats and to explore the mechanism of action of this compound. Methods: Seventy healthy female Wistar rats were randomly divided into normal group, sham operation group (intragastric administration of distilled water 10 m L · kg -1), model group (intragastric administration of distilled water 10 m L · kg -1), blood (0.03,0.02,0.01 g · kg-1) and L-NAME group (2 mg / ip) by real-time fluorescence quantitative PCR (Realtime PCR) The expression of Sirt1, p53 and NF-κB mRNA in myocardial tissue were detected. Results: Compared with the sham operation group, the expression of Sirt1 mRNA in ischemic cardiomyocytes was significantly decreased (P <0.05) and the expression of p53 and NF-κB mRNA in ischemic cardiomyocytes was increased (P <0.05). Compared with the model group, The levels of Sirt1 mRNA in ischemic cardiomyocytes were significantly increased in high, medium and low dose groups (P <0.05), and the levels of p53 and NF-κB mRNA in ischemic cardiomyocytes Significantly lower (P <0.05). Conclusion: Fufang Zhuyu Capsule can prolong the life of ischemic cardiomyocytes. The mechanism may be through activating the Sirt1 signaling pathway and inhibiting the expression of p53 and NF-κB genes.