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目的:建立以气相色谱法测定保济丸中百秋李醇含量的方法,制定并完善保济丸的质量标准。方法:采用非极性毛细管柱AT.SE-54(15m×0.25mm×0.33μm),柱温采用程序升温:起始150℃,保持23min,以8℃·min-1速率升至230℃,保持2min,进样口温度为280℃,检测器(FID)温度为280℃,载气为氮气,流速为1.0mL·min-1。结果:百秋李醇进样量在0.12～0.36μg范围内线性关系良好(r=0.9999),平均加样回收率为98.62%,RSD=4.69%。结论:该方法稳定、结果可靠、重复性好,可作为保济丸质量控制标准之一。
Objective: To establish a gas chromatographic method for determination of patchouli alcohol content in Baoji Pills, and to formulate and improve the quality standards of Baoji Pills. METHODS: The non-polar capillary column AT.SE-54 (15m×0.25mm×0.33μm) was used. The temperature of the column was increased by the program: the initial temperature was 150°C, the holding time was 23min, and the temperature was increased to 230°C at a rate of 8°C·min-1. After 2 minutes, the inlet temperature was 280°C, the detector (FID) temperature was 280°C, the carrier gas was nitrogen, and the flow rate was 1.0 mL·min-1. RESULTS: The calibration curve showed good linearity in the range of 0.12 to 0.36 μg (r=0.9999). The average recovery was 98.62% and the RSD was 4.69%. Conclusion: The method is stable, reliable, and reproducible. It can be used as one of the quality control standards of Baoji Pills.