【摘 要】
：
目的:研究当归芍药散对β淀粉样蛋白1-42(Aβ1-42)诱导的阿尔茨海默病(AD)大鼠模型的神经保护作用及对NOD样受体3(NLRP3)/半胱氨酸天冬氨酸蛋白水解酶-1(Caspase-1)信号通路的调控作用.方法:大鼠脑内注射Aβ1-42构建AD动物模型,给予不同浓度的当归芍药散治疗.实验分为假手术组,模型组,当归芍药散高、中、低剂量干预组.Morris水迷宫实验检测动物学习记忆能力;苏木素-伊红(HE)染色和高尔基染色检测神经元形态功能;免疫荧光共定位检测NLRP3炎症小体活化情况;实时荧光定量聚合
【机 构】
：
湖南中医药大学中西医结合心脑疾病防治湖南省重点实验室,长沙410208
论文部分内容阅读
目的:研究当归芍药散对β淀粉样蛋白1-42(Aβ1-42)诱导的阿尔茨海默病(AD)大鼠模型的神经保护作用及对NOD样受体3(NLRP3)/半胱氨酸天冬氨酸蛋白水解酶-1(Caspase-1)信号通路的调控作用.方法:大鼠脑内注射Aβ1-42构建AD动物模型,给予不同浓度的当归芍药散治疗.实验分为假手术组,模型组,当归芍药散高、中、低剂量干预组.Morris水迷宫实验检测动物学习记忆能力;苏木素-伊红(HE)染色和高尔基染色检测神经元形态功能;免疫荧光共定位检测NLRP3炎症小体活化情况;实时荧光定量聚合酶链式反应(Real-time PCR)检测炎症因子白细胞介素-1β(IL-1β)和IL-18 mRNA表达情况;蛋白免疫印迹法(Western blot)检测NLRP3,Caspase-1,IL-1蛋白的表达水平.结果:与假手术组比较,模型组大鼠学习记忆能力显著降低(P＜0.01);神经元形态功能受损;炎症因子IL-1β和IL-18 mRNA表达升高,NLRP3炎症小体活化增加,NLRP3,Caspase-1,IL-1β蛋白表达显著升高(P＜0.01);与模型组比较,给予当归芍药散中、高剂量干预后,AD大鼠学习记忆能力明显增加(P＜0.05,P＜0.01);神经元形态功能明显恢复;炎症因子IL-1β和IL-18 mRNA表达显著降低,NLRP3炎症小体活化减少,NLRP3,Caspase-1,IL-1β蛋白表达显著降低(P＜0.01).结论:当归芍药散可能通过调控NLRP3/Caspasc-1信号通路抑制炎症小体活化,抑制神经炎症反应,从而发挥神经保护作用.
其他文献
More than 200 million people across the world suffer from moderate to severe visual impairments,mostly due to retinal degeneration or damage to the eyes[1].Methods of reprogramming the cells in the eyes or electrical stimula-tion can be used to restore vi
Effective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases.However,ideal techniques for simultane-ously imaging neurovascular structure in a
Dear Editor,rnNeuroticism,a crucial dimension of human personality,fluctuates from emotional stability to instability[1].Compared to those with emotional stability,individuals with emotional instability show stronger attentional bias to negative stimuli,l
Acute pain plays an important physiological role as a warning system for the body.However,in many patients,nerve damage leads to neuropathic pain that can last for years and disrupts their quality of life.Unfortunately,neuropathic pain mechanisms remain l
Cell reprogramming is the process of reverting mature,specialized cells into induced pluripotent stem cells.Reprogramming also refers to the erasure and re-establish-ment of epigenetic markers during the development of mammalian germ cells,such as DNA met
Neuroimaging made it possible to quantify brain structure and function.However,there are few neuroimaging biomarkers for the early diagnosis,prognosis,and evalu-ation of therapy for brain diseases.The development of neuroimaging biomarkers for brain disea
Many recent studies have shown that the gut microbiome plays important roles in human physiology and pathology.Also,microbiome-based therapies have been used to improve health status and treat diseases.In addition,aging and neurodegenerative diseases,incl
The periaqueductal gray (PAG) is a complex mesencephalic structure involved in the integration and execution of active and passive self-protective behaviors against imminent threats,such as immobility or flight from a predator.PAG activity is also associa
Dear Editor,rnAerobic exercise is well known to improve cognitive functions including hippocampus-dependent spatial mem-ory[1]and object recognition[2].Regarding the molecular mechanisms,the indispensable role of brain-derived neu-rotrophic factor (BDNF)
儿童鼻部炎症是耳鼻咽喉科的常见病、多发病,根据发生部位不同分为鼻炎和鼻窦炎[1],其病因、症状、诊断和治疗与成人不完全相同,且容易复发,治疗困难[2].其中儿童过敏性鼻炎(allergic rhinitis,AR)、儿童急性感染性鼻-鼻窦炎、儿童过敏性鼻炎合并急性感染性鼻-鼻窦炎(混合型,MR)在儿童鼻部炎性疾病中发病率较高.我国儿童AR患病率为7.83％～20.42％;急性感染性鼻-鼻窦炎发病率为5％～6％,其中有5％～13％的儿童上呼吸道感染会导致急性鼻窦炎[3];混合性鼻炎(MR)是一种特殊的鼻炎亚