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目的 研究小卫星变异重复序列 (minisatellite variant repeat,MVR)位点 D1S8的结构多态性 ,为 DNA指纹数据库的构建及法医学应用提供基础资料。方法 应用 MVR-聚合酶链反应和聚丙烯酰胺梯度凝胶电泳银染法 ,检测 2 40名河北汉族无关个体 D1S8位点重复序列的变异并进行数字编码。结果每一个体得到约 30个数字编码 ,未发现任何两个无关个体编码相同 ,30个编码完全相同的概率为 3.5 5×10 - 1 1 。 3种重复单位 a-型、t-型和 o-型出现的比例分别为 5 4.77%、42 .5 4%和 2 .6 9%。该位点杂合度为0 .9837,非父排除率为 0 .96 6 9,多态性信息含量为 0 .9833。结论 D1S8位点多态性程度高 ,在法医学个人识别和亲子鉴定中具有重要的应用价值
Objective To study the structural polymorphism of D1S8 in minisatellite variant repeat (MVR) locus and provide basic information for the construction of DNA fingerprinting database and forensic application. Methods MVR-PCR and polyacrylamide gradient gel electrophoresis silver staining were used to detect the variation of D1S8 repeats in 240 unrelated individuals in Hebei Han and were digitally coded. As a result, about 30 numbers were obtained for each individual, and no two unrelated individuals were found to have the same coding rate. The probability of 30 identical codes was 3.5 5 × 10 - 1 1. The proportions of a-type, t-type and o-type of the three kinds of repeat units were 5 4.77%, 42.54% and 2.69% respectively. The heterozygosity at this locus was 0.9837, the non-parent exclusion rate was 0.96 6 9, and the polymorphism information content was 0.9833. Conclusion There is a high degree of polymorphism at D1S8 locus, which is of great value in personal identification and paternity testing of forensic medicine