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以银纳米粒子自组装层为增强基底,我们报道了一种用于检测二元磷脂膜中具有相似结构磷脂分布的表面增强拉曼成像方法,这种方法具有免标记及花费低廉的优点.对探针分子对巯基苯胺(p-aminothiophenol),实验中所用的银纳米粒子自组装层表现出强的表面增强拉曼活性及良好的重现性.原子力显微镜表征结果证明了完整的磷脂膜在银纳米粒子自组装层上的形成.以这种银自组装层为基底,我们得到了磷脂膜中二肉豆蔻酰磷脂酰甘油(DMPG)和二肉豆蔻酰磷脂酰胆碱(DMPC)的表面增强拉曼光谱,并且利用DMPG的光谱特征峰,1482cm-1,区分这两种磷脂.而通过1482cm-1和1650cm-1的峰强比(R1482/1650),可以同时得知在混合磷脂膜上某点这两种磷脂所占的比例:R1482/1650值的增加意味着DMPG的增加和DMPC的减少.磷脂膜的表面增强拉曼成像则是由R1482/1650值和对应的位置信息组合而得到,其成像结果表明了带电的磷脂DMPG在混合磷脂膜中的聚集.我们所报道的基于表面增强拉曼成像技术的方法提供了一种便利的、免标记的和花费低廉的途径来研究磷脂膜的结构,例如磷脂域和脂阀.
We have reported a surface-enhanced Raman imaging method for detecting the distribution of phospholipids with similar structure in binary phospholipid membranes by using silver nanoparticle self-assembled layers as the reinforcing substrate. This method has the advantages of free labeling and low cost. The probe molecule showed strong surface-enhanced Raman activity and good reproducibility for the p-aminothiophenol, the silver nanoparticle self-assembled layer used in the experiment.Analytic results by atomic force microscopy demonstrated that the intact phospholipid membrane was in silver Nanoparticle Self-Assembly Layer Formation Using this silver self-assembled layer as a substrate, we obtained the surface enhancement of dimyristoylphosphatidylglycerol (DMPG) and dimyristoylphosphatidylcholine (DMPC) in the phospholipid membrane Raman spectroscopy, and DMPG spectral characteristic peak, 1482cm-1, to distinguish between the two phospholipids by 1482cm-1 and 1650cm-1 peak intensity ratio (R1482 / 1650), can also be found in the mixed phospholipid membrane The ratio of these two types of phospholipids at a certain point: an increase in the R1482 / 1650 value means an increase in DMPG and a decrease in DMPC. Surface enhanced Raman imaging of the phospholipid membrane results from the combination of the R1482 / 1650 value and the corresponding position information , Its imaging junction Indicating the aggregation of charged phospholipid DMPG in a mixed phospholipid membrane.The method we reported reported based on surface enhanced Raman imaging provides a convenient, label-free and cost-effective way to study the structure of phospholipid membranes such as Phospholipid domains and lipid valves.