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目的:利用牙周细胞体外创伤模型,探讨MTA、银汞合金和IRM对人牙周韧带细胞生长的影响。方法:在培养皿中形成人牙周韧带细胞的融合培养物,以机械方法去除宽3mm的细胞层条带,形成体外创伤模型。在受损培养物中,分别加入以上3种根尖倒充填材料的浸提液,并以含有10%胎牛血清的培养液作为阳性对照组,含有0.1%胎牛血清的培养液作为阴性对照组。继续培养2、6、9d,对每个时间点细胞增殖能力和对创面的覆盖能力进行检测,应用SPSS11.5软件包对数据进行统计学分析。结果:第2天时,细胞增殖能力为MTA组>银汞合金组≈IRM组;第6天和第9天时,细胞覆盖能力为MTA组>银汞合金组>IRM组(P<0.05)。结论:3种材料中,MTA的生物相容性最好,其次是银汞合金,而IRM最差。
OBJECTIVE: To investigate the effects of MTA, silver amalgam and IRM on the growth of human periodontal ligament cells using in vitro wound models of periodontal cells. Methods: A fusion culture of human periodontal ligament cells was formed in a petri dish and a 3 mm wide cell layer strip was mechanically removed to form an in vitro wound model. In the damaged culture, the extracts of the above three kinds of root tip inverted filling materials were respectively added, and the culture solution containing 10% fetal calf serum was used as the positive control group and the culture solution containing 0.1% fetal bovine serum was used as the negative control group. The cells were cultured for 2, 6 and 9 days. The cell proliferative ability and coverage of the wounds were detected at each time point. SPSS11.5 software package was used to analyze the data. Results: On day 2, the cell proliferation ability was MTA group> amalgam group ≈IRM group. On the 6th day and the 9th day, the cell coverage was MTA group> amalgam group> IRM group (P <0.05). Conclusion: Among the three materials, MTA has the best biocompatibility, followed by amalgam, while IRM has the worst IRM.