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目的观察肝癌小鼠模型射频治疗后外周血肿瘤坏死因子-α(TNF-α)和白细胞介素-2(IL-2)的变化及癌组织 CD54的表达情况,探讨肝癌射频治疗对机体免疫功能的影响。方法 Balb’s小鼠60只,随机分为2组,对照组(C 组)和射频治疗组(R 组),每组30只。成功制作小鼠肝细胞癌模型后,R 组给予射频治疗,并于其后0h、2h、2周、3周、4周处死并眼球取血法取血,采用双抗体夹心 ELISA 法测定 TNF-α和 IL-2光密度值(OD),同时切除肿瘤进行免疫组化 CD54表达分析。结果R组肿瘤明显缩小,其 TNF-α和 IL-2浓度呈递增趋势,与对照组比较差异有显著性(P<0.05);经射频处理后的 R 组肝癌细胞 CD54呈过度表达现象,并与各时间段 TNF-α、IL-2浓度呈明显正相关(r=0.520,P<0.01;r=0.501.P<0.01)。结论肝癌射频治疗诱使 TNF-α、IL-2浓度增高,并上调细胞间粘附分子 CD54的表达,从而诱导肿瘤免疫应答而发挥抗肿瘤作用。
Objective To observe the changes of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) and the expression of CD54 in peripheral blood of mice with hepatocellular carcinoma after radiofrequency ablation and to investigate the effect of radiofrequency treatment on immune function Impact. Methods Sixty Balb’s mice were randomly divided into two groups: control group (C group) and radiofrequency treatment group (R group), 30 rats in each group. After the model of mouse hepatocellular carcinoma was successfully established, R group was treated with radiofrequency ablation and was sacrificed at 0h, 2h, 2 weeks, 3 weeks and 4 weeks later. Blood samples were obtained from the eyeball. The serum levels of TNF- α and IL-2 optical density (OD), and excised the tumor for immunohistochemical analysis of CD54 expression. Results The tumors in group R were significantly reduced, and the levels of TNF-α and IL-2 showed an increasing trend compared with those in control group (P <0.05). CD54 in group R was overexpressed There was a significant positive correlation between TNF-α and IL-2 concentrations at each time point (r = 0.520, P <0.01; r = 0.501, P <0.01). Conclusion The radiofrequency ablation of liver cancer induces the increase of TNF-α and IL-2, and up-regulates the expression of intercellular adhesion molecule CD54, thereby inducing tumor immune response and exerting anti-tumor effect.