利用甲基磺酸乙酯(ethyl methane sulfonate,EMS)、叠氮化钠(sodium azide,NaN3)和N离子束分别诱变处理大豆品种“冀黄13”的种子。经M2选择,M3、M4代验证,共筛选出茎器官突变体15份,叶器官突变体19份,花器官突变体31份,种子器官突变体3份以及成熟期突变体18份,其中有9份突变体属于复合性状的突变,最终获得77份稳定遗传的突变体。通过F2群体的表型分析,确定1份雄性不育突变体和1份黑种皮色突变体的表型均由单隐性基因控制。本研究所获得的突变体和所构建突变体库将为大豆功能基因组学研究和遗传改良奠定基础。 The seeds of soybean variety “Jihuang 13” were mutagenized with ethyl methane sulfonate (EMS), sodium azide (NaN3) and N ion beam respectively. After M2, M3 and M4 generation tests, 15 mutants were screened out, 19 organ of leaf organ, 31 floral organ, 31 organ of seed and 18 mutants of maturity Nine mutants belonged to the composite trait and finally obtained 77 stable inherited mutants. Phenotypic analysis of the F2 population determined that the phenotypes of one male sterile mutant and one black mutant were controlled by a single recessive gene. The mutants and the constructed mutants obtained in this study will lay the foundation for soybean functional genomics research and genetic improvement.