论文部分内容阅读
目的:观察急性早幼粒细胞白血病(APL)细胞来源微粒(APL-MP)的促凝活性、表面组织因子(TF)表达情况、TF在其促凝活性中发挥的作用及分化治疗药物三氧化二砷(ATO)对上述指标有何影响。方法:选取3例初发APL患者,提取骨髓APL细胞,3名缺铁性贫血患者提取骨髓单个核细胞作为对照。分别用不同浓度ATO处理APL细胞24 h、48 h、72 h,收集细胞培养液提取微粒。采用流式细胞术对微粒进行定量分析并进行微粒表面TF表达情况检测;利用凝血实验比较不同组细胞释放微粒的促凝血活性;应用抗TF抗体抑制微粒促凝血活性实验检测TF在APL-MP的促凝血活性中发挥多大作用。结果:1.0μM及2.0μM ATO能显著促进APL细胞释放微粒。与正常骨髓来源单个核细胞释放的微粒相比,骨髓APL-MP的TF表达及促凝活性均显著增高,0.5μM及1.0μM ATO处理可以有效降低APL-MP的TF表达及促凝活性,且这一作用呈时间依赖性。各组APL-MP经抗TF抗体孵育后凝血时间显著延长。结论:APL-MP的TF表达和促凝学活性均显著增高,并且TF在APL-MP的促凝血活性中发挥着重要作用。ATO能显著促进APL细胞释放微粒,低浓度ATO可以有效降低APL-MP的TF表达及促凝血活性。
OBJECTIVE: To observe the procoagulant activity, surface tissue factor (TF) expression in APL-derived cells (APL-MP), the role of TF in its procoagulant activity and the therapeutic effect of arsenic trioxide ATO) the impact of these indicators. Methods: Three APL patients with newly diagnosed APL were selected, bone marrow APL cells were extracted and three patients with iron deficiency anemia were selected as control. APL cells were treated with different concentrations of ATO for 24 h, 48 h and 72 h, respectively, and the cell culture medium was collected to extract the microparticles. Flow cytometry was used to quantitatively analyze the particles and detect the expression of TF on the surface of the particles. The coagulation assay was used to compare the procoagulant activity of the released particles in different groups of cells. The anti-TF antibody was used to detect the activity of TF in APL-MP Promote the role of procoagulant activity. Results: 1.0μM and 2.0μM ATO can significantly promote the release of APL cells particles. Compared with normal bone marrow-derived mononuclear cells, TF expression and procoagulant activity of APL-MP in bone marrow were significantly increased. At 0.5μM and 1.0μM ATO treatment, the TF expression and procoagulant activity of APL-MP were significantly decreased This effect is time-dependent. The clotting time of APL-MP in each group after anti-TF antibody incubation was significantly prolonged. CONCLUSION: TF expression and procoagulant activity of APL-MP are significantly increased, and TF plays an important role in the procoagulant activity of APL-MP. ATO can significantly promote the release of APL cells particles, low concentrations of ATO can effectively reduce APL-MP TF expression and procoagulant activity.