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利用冷冻真空干燥技术保存菌种,这是早已被实践证实的长期保存菌种的有效方法。但此法需一定的设备和条件,因而不能被基层广泛采用。为适於基层的菌种保管工作,我们设计了纸片菌种干燥法,於1970～1972年先后干燥了一批菌种,观察到1980年5月仍存活,且生物学特性也未改变,现介绍如下:一、材料和方法(一)保存剂:将新鲜牛奶以流通蒸汽消毒30分钟,冷却后放4℃冰箱过夜。脱脂后,
The use of freeze-drying techniques to conserve bacteria is an effective method of long-term preservation of bacteria that has long been proven. However, this method requires certain equipment and conditions and therefore can not be widely adopted by grassroots units. In order to adapt to the custody of grass-roots bacteria, we designed a paper-based bacteria drying method. A series of bacteria were dried successively from 1970 to 1972, and it was observed that they survived in May 1980 with no change in biological characteristics. Now introduced as follows: First, materials and methods (A) Preservatives: The fresh milk to steam for 30 minutes, cooled and put 4 ℃ refrigerator overnight. After degreasing,