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目的通过对不同分化阶段的兔肌成纤维细胞及前体施加不同的基底应变刺激,研究此过程中细胞表达α-SMA的时间剂量关系。方法选取异体蛋白植入法获得5 d和7 d的肌成纤维细胞(前体),培养于弹性膜上;利用基底静态拉伸对其分别施以应变从0%～3%和3%～6%的分段载荷及0%～6%的阶跃式载荷。结果 3 d阶段的肌成纤维细胞(前体)没有表达α-SMA;5～7 d阶段可以监测到α-SMA的表达;在10～15 d阶段则表达显著增强(P<0.01)。两种加载方式下,α-SMA的表达量均显著增加;分段加载作用下SMA的表达量更高。结论肌成纤维细胞(前体)α-SMA受基底静态拉伸后表达增加,且对不同的加载方式能做出不同的响应,提示在肌成纤维细胞的分化和愈伤反应过程中,力学因素扮演着十分重要的角色。
OBJECTIVE: To study the time-dose relationship of α-SMA expression during different differentiation stages of rabbit myofibroblasts and precursors by applying different basal strain stimulation. Methods The myofibroblasts (precursors) were obtained on the 5th and 7th day after implantation of allogeneic protein and cultured on the elastic membrane. Strain from 0% to 3% and 3% 6% of the segmented load and 0% ~ 6% of the step load. Results α-SMA was not expressed in myofibroblasts (precursors) at 3 d stage. The expression of α-SMA was observed at 5 to 7 days and significantly increased at 10 to 15 days (P <0.01). The expression of α-SMA increased significantly under the two loading methods; the expression of SMA was higher under the segmental loading. Conclusion The α-SMA of myofibroblasts (precursors) is increased by static stretching of the basal lamina, and different responses can be made to different loading methods, suggesting that in the process of myofibroblast differentiation and callus induction, mechanics Factors play a very important role.