论文部分内容阅读
目的研究幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）空泡毒作用。方法用体外细胞毒试验，结合细胞化学及荧光染色、透射电镜技术研究Ｈｐ空泡毒作用及其病理机制。结果７８．２６％的Ｈｐ相关消化性溃疡患者感染的是空泡毒作用阳性Ｈｐ（Ｔｏｘｉｎ＋），而只有４２．８６％的胃炎患者感染的是Ｈｐ（Ｔｏｘｉｎ＋）。Ｈｐ（Ｔｏｘｉｎ＋）引起的细胞空泡样变，吖啶橙及酸性磷酸酶染色阳性；透射电镜显示空泡为双层膜结构。结论Ｈｐ（Ｔｏｘｉｎ＋）感染与Ｈｐ相关消化性溃疡密切相关。Ｈｐ（Ｔｏｘｉｎ＋）引起的空泡是自噬体，是细胞在毒性物质作用下表现的病理现象。
Objective To study the vacuolar toxicity of Helicobacter pylori (Hp). Methods In vitro cytotoxicity assay combined with cytochemistry and fluorescent staining, transmission electron microscopy was used to study the role of Hp vacuolization and its pathological mechanism. Results 78.26% of Hp-related peptic ulcer patients were infected with vacuole-positive Hp (Toxin +), while only 42.86% of patients with gastritis were infected with Hp (Toxin +). Hp (Toxin +) induced vacuolar degeneration, acridine orange and acid phosphatase staining positive; transmission electron microscopy showed vacuoles to double membrane structure. Conclusion Hp (Toxin +) infection is closely related to Hp-related peptic ulcer. Hypoxemia induced by Hp (Toxin +) is an autophagosome that is a pathological phenomenon in which cells behave under toxic substances.