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目的建立高效毛细管电泳法分离尼卡地平、氧氟沙星、卡维地洛、佐匹克隆和普萘洛尔5种碱性药物对映体。方法采用正交试验设计法,以羧甲基-β-环糊精(carboxymethyl-β-cyclodextrin,CM-β-CD)为手性选择剂,使用未涂层熔融石英毛细管柱,以NaH2PO4缓冲溶液为背景电解质,运行电压为20 kV。结果在最佳条件下,尼卡地平、氧氟沙星、卡维地洛、佐匹克隆和普萘洛尔的对映体分离度分别为3.8、9.8、6.2、5.7和5.0。结论 CM-β-CD对尼卡地平等5种手性药物有较高的对映体选择性。该方法简便、高效、灵敏、重现性好。
OBJECTIVE To establish a high performance capillary electrophoresis method for the separation of five basic enantiomers of nicardipine, ofloxacin, carvedilol, zopiclone and propranolol. Methods The orthogonal design was used to investigate the effect of carboxymethyl-β-cyclodextrin (CM-β-CD) as a chiral selector on the surface of uncoated fused silica capillary column As the background electrolyte, the operating voltage is 20 kV. Results Under optimal conditions, the enantiomeric separations of nicardipine, ofloxacin, carvedilol, zopiclone and propranolol were 3.8, 9.8, 6.2, 5.7 and 5.0, respectively. Conclusion CM-β-CD has good enantioselectivity for five chiral drugs like nicardipine. The method is simple, efficient, sensitive and reproducible.