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The PSV·NS3, an expressing recombinant plasmid of dengue virus type 2 NS3 protein, was injected directly into the quadriceps of Balb/C mice to explore whether it could inducing immune response. The splenic T cell subsets of two groups was analysed by flow cytometry . It was found that the percentage of CD4 + and CD8 + T cells of experimental group were significantly higher than those of the control group .The titer of IgG antibody was as high as 1:5 120 in experimental group, but it couldn’t be detected in control group by ELISA. The western blot further proved that the IgG antibody was specific for NS3 protein. Those results Suggested that inoculation Balb/C mice with PSV·NS3 could inducing immune response, and the NS3 protein might be used as the candidate protein of DNA vaccine of dengue virus.
The PSV · NS3, an expressing recombinant plasmid of dengue virus type 2 NS3 protein, was injected directly into the quadriceps of Balb / C mice to explore whether it could inducing immune response. The splenic T cell subsets of two groups was analysed by flow cytometry It was found that the percentage of CD4 + and CD8 + T cells of experimental group were significantly higher than those of the control group. The titer of IgG antibody was as high as 1: 5 120 in experimental group, but it could not The results of the inoculation of Balb / C mice with PSV · NS3 could inducing immune response, and the NS3 protein might be used as the candidate protein of DNA vaccine of dengue virus.