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应用全细胞膜片钳技术，研究乙氧异黄酮二酸钠（ｅｔｈｏｘｙ－ｉｓｏｆｌａｖｏｎｅ２－ｃａｒｂｏｘｙｌｉｃａｃｉｄ，ＥＩＣＡ）对豚鼠心室肌细胞上慢钙电流（Ｉｃａ）的影响。结果发现ＥＩＣＡ能使心室肌细胞Ｉｃａ呈剂量依赖性降低，阈浓度为１０－８ｍｏｌ／Ｌ，最大有效浓度为３×１０－５ｍｏｌ／Ｌ；１０－６ｍｏｌ／ＬＥＩＣＡ可明显阻断异丙肾上腺素（Ｉｓｏｐｒｅｎａｌｉｎｅ，Ｉｓｏ）和钙通道激动剂ＢａｙＫ８６４４所致的Ｉｃａ增加（Ｐ＜０．０１）。ＥＩＣＡ能增加外向电流（Ｌｏｕｔ），并被四乙基胺（Ｔｅｔｒａｅｔｈｙｌａｍｍｏｎｉｕｍ，ＴＥＡ，１０－６ｍｏｌ／Ｌ）所拮抗。结果提示：ＥＩＣＡ的降压、减慢心率、抗心律失常作用机制主要与阻断Ｃａ２＋通道（Ｌ型）有关。
Whole-cell patch-clamp technique was used to study the effects of ethoxy-isoflavone 2-carboxylic acid (EICA) on slow calcium current (Ica) in guinea pig ventricular myocytes. The results showed that EICA could decrease the Ica of cardiomyocytes in a dose-dependent manner, the threshold concentration was 10-8mol / L, the maximum effective concentration was 3 × 10-5mol / L; 10-6mol / LEICA could obviously block isoproterenol Isoprenaline, Iso) and Ikca of calcium channel agonist BayK8644 increased (P <0.01). EICA increased the outward current (Lout) and was antagonized by tetraethylammonium (TEA, 10-6 mol / L). The results suggest that: EICA antihypertensive, slow heart rate, anti-arrhythmia mechanism mainly blocking the Ca2 + channel (L type).