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文章简介持留菌是指细菌在不产生基因变异的情况下能够耐受抗生素的个体。以往的研究认为持留菌受多个信号通路调控,而细胞沉默是产生持留菌的主要机制,但是抗生素是否能够进入持留菌细胞内尚不清楚。本研究利用荧光抗生素和高分辨荧光成像系统观察到抗生素能够在持留菌中积累,但积累量明显低于敏感细胞中的水平。紧接着用转录组测序的方法分析持留菌中基因
Introduction Bacteria are bacteria that are able to tolerate antibiotics without causing genetic variation. Previous studies suggested that the retention of bacteria by a number of signaling pathways, and cell silence is the main mechanism to produce retention bacteria, but whether antibiotics can enter the cells holding bacteria remains unclear. In this study, antibiotics were observed to be able to accumulate in retention bacteria using fluorescent antibiotics and high-resolution fluorescence imaging systems, but the accumulation was significantly lower than that in sensitive cells. Following the transcriptome sequencing method, the genes in the retained bacteria were analyzed