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代谢组学是通过考察生物体系受刺激或扰动前后(如某个特定的基因变异或环境变化后)代谢产物的动态变化,研究生物体系代谢网络的一种技术,其研究对象主要是内源性小分子物质。这些物质大部分极性强,难挥发,在气相色谱-质谱分析前,需要进行适当的化学衍生处理。近几年来化学衍生技术发展迅速,本文主要从衍生试剂种类、衍生条件和衍生效果(包括衍生效率、重复性和产物稳定性等)出发,综述了已报道的衍生方法的特点,并展望了衍生技术的发展前景。
Metabolomics is a technique for studying metabolic networks of biological systems by examining the dynamic changes of metabolites in biological systems before or after stimulated or perturbed (eg, after a particular gene mutation or environmental change), and its research object is mainly endogenous Small molecule substances. Most of these substances are very polar and less volatile and require proper chemical derivatization prior to GC-MS analysis. In recent years, the rapid development of chemical derivatization technology, this article mainly from the derivative reagents, derivatization conditions and derivatization effects (including derivatization efficiency, repeatability and product stability, etc.), reviewed the characteristics of the reported derivative methods, and prospects of derivatives Technology development prospects.