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目的探讨复方丹参药物血清对入胃癌细胞增殖的抑制作用。方法给予体外培养人胃癌细胞复方丹参药物血清,用克隆形成法和流式细胞仪检测对细胞分裂增殖和细胞凋亡的影响。结果复方丹参药物血清(3.6、7.3、15g/kg)和5-氟脲嘧啶(5-FU)组克隆数比对照组克隆数(78.67±2.2)明显减少,克隆形成抑制率分别为13.1%、46.6%、64.0%和40.7%.复方丹参药物血清(3.6、7.3、15g/kg)组和5-FU凋亡率比对照组凋亡率(7.4%)明显增加,分别为:16.3%、24.2%、31.1%和22.1%。结论复方丹参药物血清对人胃癌细胞分裂增殖具有明显的毒性和抑制作用。
Objective To investigate the inhibitory effect of Compound Salvia Miltiorrhiza on the proliferation of gastric cancer cells. Methods The human gastric cancer cells were cultured in vitro, and the effect of compound Salvia miltiorrhiza bunge drug serum on cell division, proliferation and apoptosis was detected by clone formation assay and flow cytometry. Results The number of clones of Fufang Danshen serum (3.6, 7.3, 15g / kg) and 5-fluorouracil (5-FU) group was significantly lower than that of the control group (78.67 ± 2.2). The inhibition rates of cloning and formation were 13.1% 46.6%, 64.0% and 40.7% respectively.The apoptotic rates of the compound Danshen serum (3.6, 7.3, 15g / kg) and 5-FU group were significantly higher than those of the control group (7.4% %, 31.1% and 22.1%. Conclusion The compound salvia miltiorrhiza compound serum has obvious toxicity and inhibition on human gastric cancer cell division and proliferation.